MY SCIENTIFIC ACCOMPLISHMENTS
To the best of my knowledge to date I was the first:
1) First detection of a p53 mutation in a human medulloblastoma.
My findings on medulloblastomas were published together with Hiroko's data on oligodendrogliomas: Ohgaki, Eibl, et al. CANCER RESEARCH 1991. (Note: at the same time another, the Vogelsetein-group published another p53 mutation in a medulloblastoma cell line, i.e. not within a primary medulloblastoma - therefore I consider me as the very first for original tumor tissue)
2) First detection of a high (50%) frequency of p53 mutations in low-grade astrocytomas (WHO grade II), as well as in anaplastic astrocytomas (WHO grade III) and in Glioblastoma multiforme (GBM WHO grade IV).
These findings were reproduced under my supervision by my trainee from Harvard Medical School and later combined with other (expected) data on the chromosomal loss of the arm harbouring the p53 gene (von Deimling, Eibl, et al. CANCER RESEARCH 1992). I'm not sure if anyone else before myself also found the high incidence of p53 mutations in low-grade astrocytomas (WHO grade II). Since I never wanted to become full professor of neuropathology I didn't fight for a first authorship of my very first finding of these mutations in the low-grade astrocytomas. I also could not detect any p53 mutations in any of the pilocytic Astrocytomas (similar name, but different biology compared to other astrocytic tumors), but since my supervised guest published our results in Cancer Research, I could not publish the same material as original paper anywhere else.
3) Development of a brain tumor model for PNET (primitive neuroectodermal tumors/medulloblastomas), histologically indistiguishable from human medulloblastoma. First published Abstract: Eibl and Wiestler 1991; Original Paper several years later: Eibl et al. 1994: American Journal of Pathology - With presenting this model in Bonn, O.D. Wiestler became immediately full professor. He continued and was able to reproduce this model in Bonn, where there was already an an established research group in the field of SV40 large T-antigen, which induced the tumors in my model (with p53 as one known interactions partner of SV40LT).
4.1) First detection of CD44v splice variants in human brain tumors (CD44v found on RNA and protein level, histochemistry, as well as cell sorting and functional assays; Experiments in Karlsruhe) - at the same time others in my lab published with collaborators just the opposite (i.e. they were unable to detect splice variants in such tumors). Their methods and/ their work was not sensitive or accurate enough. I could perform even functional assays on living cells...
4.2) Genomic organisation of CD44 gene and its variant exons and the end of CD44 gene (mouse library was SV129 strain, unusual, but necessary for isogenic K.O. constructs). Interestingly, this was confirmed and published with only acknowledging me as 'results confirmed by Eibl', without my Southern-Blot findings the length of an intron of several kb could not be detected by PCR as suggested.
5) Unpublished: Tumor cell rolling under shear as a model for organ-specific metastasis. Model for melanoma metastasis. (Experiments in Eugene C. Butcher lab, Stanford University; officially sponsored by Irv Weissman)
6) Unpublished: Physiologic monocyte rolling and CD44 functional experiments/regulation (Collaboration with Dr. Marcus Hubbe, Eugene C. Butcher-lab, Stanford University)
7) First AFM measurment of specific mammalian cell-cell interactions on a single-molecule level. First measurement of VLA-4 / VCAM-1 interaction with AFM and at the single molecule level on living cells (Eibl and Benoit 2004). Experiments started 2001 with Martin Benoit in Hermann E. Gaub - Biophysics lab in Munich, supported with a chemical integrin inhibitor from Horst Kessler (Chemistry, Munich)... I could get very best recommendations from both Professors (and a third collaboration partner, formerly at Harvard) for my 50 pages grant application in the USA, but then the Munich lab started to get my project going in Munich - resulting in several highly questionable master and doctorate theses ...
8) First AFM measurment of activation (modulation) of any integrin by any chemokine on any cell and at the single-molecule level: VLA-4 / SDF-1 / VCAM-1 (Munich and Miami).
(unfortunately, this Nobel-like experiment was overtaken by my collaborators)
9) Unpublished: 2001 Detection of CXCR4 and CCR7 chemokine receptor espression in all types of breast cancer as well as in normal and premalignant mammary gland tissue. (Findings were in contrast to a Nature paper in 2001 by Albert Zlotnik's group). Other chemokine receptors expressed in malignant tissues.
here are just a few of my Fotos (click here), including several pics and a video of Apollo butterflies (Parnassius apollo) from different spots in Bavaria/Germany (otherwise extinct in most parts of Germany), as well as pics of a totally black snake (melanism), orchids and other wildlife in bavaria
if the link doesn*t work: http://www.flickr.com/photos/eibl
My moderate (!) LETTER TO THE EDITOR 06/2008
My following, and as I think, really moderate letter points to clear mistakes in a review of German biophysisists - which is not corrected by Fiona Watt, Editor in chief of the Journal of Cell Science.
All three of my points have been completely confirmed by independent scientific experts, but none of the critisized authors provided a publishable reply, nor did they support the publicaton of a "correcting addendum" to their clear mistakes:
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Single-receptor adhesion measurements on living cells
Helenius et al.(1) [Helenius, Heisenberg, Gaub HE, Müller] review the use of atomic force microscopy (AFM) for single-cell force spectroscopy (SCFS). In Table 1 of their report, they list the references for ‘receptor-ligand interactions by SCFS using living cells as probes’. These references include two papers detecting the rupture force of individual cell adhesion bonds of integrin alpha4beta1 (VLA-4) on cells to its ligand VCAM-1. Surprisingly, however, Helenius and co-workers have not included the work of Eibl and Benoit (2) in their commentary, although the findings concerning the same integrin to ligand interaction were published 5 and 18 months, respectively, before the two cited references appeared. In addition, Table 1 contains two smaller mistakes. First, the work of Thie et al.(3) is used as a reference for the specific measurement of LFA-1 (integrin alphaLbeta2) on its ligand ICAM-1, but these authors never claimed to specifically measure any cell adhesion receptor. To the contrary, they state that they could only speculate regarding the cell adhesion receptors involved, which might include several integrins and other cell adhesion receptors. This citation may also mislead readers with regard to several aspects of the technique for measuring leukocyte homing receptors with AFM at the single-molecule or single-receptor level, including the original developers of the approach and the time-frame in which it was developed. Second, Table 1 also includes a repeated typing error: concavalin A instead of concanavalin A. In my view, a detailed step-by-step protocol in this area could have been included in the review, too (4). For readers interested in an extensive overview of this topic, a book chapter is soon to be published that will also review this subject and include a similar table as well as further protocols for experiments (5).
(1) Helenius, J., Heisenberg, C.P., Gaub, H.E., Muller, D.J. (2008). Single-cell force spectroscopy. J. Cell. Sci. 121, 1785-91
(2) Eibl, R.H. and Benoit, M. (2004). Molecular resolution of cell adhesion forces. IEE - Nanobiotechnology 151, 128-132
(3) Thie M, Röspel R, Dettmann W, Benoit M, Ludwig M, Gaub HE, Denker HW (1998). Interactions between trophoblast and uterine epithelium: monitoring of adhesive forces. Hum Reprod. (11):3211-9
(4) Eibl, R.H. and Moy V.T. (2005). Atomic force microscopy measurements of protein-ligand interactions on living cells. In: Protein-Ligand Interactions. (Editor: G.Ulrich Nienhaus), Humana Press, Totowa, NJ, U.S.A., pp. 437-448 ISBN 1588293726
(5) Eibl, R.H. (in press). Direct force measurements of receptor-ligand interactions on living cells. In: Applied Scanning Probe Methods. Bhushan, B., Fuchs, H., Tomitori, M. (editors), Springer, Heidelberg
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In 2002 I obtained a governmental stipend from DFG, Bonn (German research foundation) to meet with Harvard Professor Timothy A. Springer (Crafoord prize winner 2004; equivalent to Nobel prize) in the USA in order to discuss my extremely pioneering projects as a project leader between nanotechnology and immunology, i.e. AFM force measurements of VLA-4 integrin on living lymphocytes and possibly the activation by SDF-1, a project I had planned and developed already since May 2001 !!! Later, I was able to have Professors from Germyany submitting letters of reference to him, including a letter of reference from a famous Professor of Biophysics. Unfortunately, until today Tim Springer himself, as well as the President of Harvard University avoid to confirm any of these facts - and, unfortunately, are not helpful in any way in my fight against severest plagiarism of my life's work. Please note: I don't accuse Tim Springer himself, nor any of his current lab members of plagiarising any of my projects of measuring with AFM on the single molecule level VLA-4 / VCAM-1 interactions on a living cell.
If I remember correctly, the Harvard University is known to be the University of the three lies - for historical reasons, but I wonder how it will be called in the future due to this scientifically very questionnable behaviour.
Interestingly, the University of Munich including its president also tries to avoid the written confirmation of the originality of my project and also my project leadership on measuring VLA-4 (and other homing receptors I was working on long before I shared my knowledge with the University) on living cells (including lymphocytes) with AFM at the single-molecule level and the activation measurements by SDF-1.
older NEWS Dec. 2008:
1. Inclusion in Marquis' "Who's Who in Medicine and Healthcare", 2009
2. Robert Eibl on network.NATURE.com http://network.nature.com/people/U81AA8F85/profile
3. INVITED TALK(German Society of Immunology / 35th Annual Meeting of Clinical immunology, Frankfurt am Main): "Homing: comparison of lymphocytes and metastases"
4. My BOOK CHAPTER (31 pages !!!) appears on Dec. 1st , 2008 in the USA (whole book costs about 199 USD/139.05 EUR) Robert H. Eibl: Direct Force Measurements of Receptor–Ligand Interactions on Living Cells In: Applied Scanning Probe Methods XII - Characterization. Bhushan B, Fuchs H (Editors), Springer, Heidelberg, pp. 1-31, (2009)
5. Click here to view my public profile on ResearcherID.com
NEWS: inclusion in Marquis'
"Who's Who" in the World
I should mention that I don't know who nominated me for this inclusion. PLEASE note, I never bought anything from Marquis' Who'swho, nor did I pay anything to be included, nor did I nominate myself in any way.
Here some minor or funny prizes I won in 2008:
1) One of the two major weekly journals in Germany (Focus) awarded me with a prize for a funny, but potential excuse, which a German museum could have used earlier in 2008. It became the victim of chinese plagiates and therefore had to close its major exhibition of chinese warriors. My comment is in German language, it mainly critisizes the naive German willingness to beleive everything from anyone - of course, my satirical comment is not intended in any way to critisize chinese culture - we all know nobody could have expected to get the real chinese warriors to Germany, especially by a company not representing Chinese government)
2) I've been awarded from the WDR (large public TV-broadcast) for my published gardeners' tip. I received a valuable book.
Nature-online published a Commentary of Robert Eibl on the Nobel prize in the field of Embryonic stem cells and K.O. mice ( http://www.nature.com/news/2007/071010/full/449642a.html )
Another comment in 2008 on BSE you will find here, in Nature.com : ( http://www.nature.com/news/2008/080701/full/news.2008.926.html?q=2#last-comment )
As many of you know I'm the first scientist measuring with AFM-based single-molecule force spectroscopy the physiologic activation of any integrin by any chemokine on any cell type. Major molecules of interest: VLA-4, VCAM-1, SDF-1 - but since the beginning of this project in 2001, any measurement of (rapid) regulation of cell adhesion and the molecular resolution of it came into the focus of my independend research approaches..
A propos, recent (and expected) future Nobel prizes for Stanford University:
In 2006, two Nobel prizes were given to researchers at Stanford Medical School - I expect more in the coming years.
1) Nobel prize in Physiology and Medicine: Andrew Fire (Pathology and Genetics)
2) Chemistry: Roger Kornberg (Structural Biology)
- still remembering his very clear lecture and discussion with him I sent congratulations.
Thanks to Stanford to accept my second comment:
".... the comments of Robert Eibl, Bad Reichenhall, Germany, October 9, 2006 04:33 AM
As a former postdoc in Weissman lab, I expected at least one Nobel prize to go to the Stanford University School of Medicine in the coming years. I remember a great lecture of and a short discussion with Roger Kornberg during my years at Stanford. This prize is sure a wonderful and great honor for him and his work, but also for the 'little' Stanford School of Medicine receiving even two prizes in one week (Andy Fire for Medicine and Roger Kornberg for Chemistry). For me it was a great honor to improve my scientific education and serve the School of Medicine as a postdoc. Congratulations to the Nobelist !"
Irving L. Weissman (M.D.)
my official sponsor at Stanford University - except the Nobel, many intl. awards
Eugene C. Butcher (M.D.)
he provided the lab equipment I needed for my independent research concept on VLA-4/VCAM-1 and living cells; he received the swedish Crafoord prize 2004 which is the only official equivalent to the Nobel prize (a former member of Weissman lab)
Paul Kleihues (M.D.)
former director of IARC, Lyon; former Head of Neuropathology, Univ. Zürich
Otmar D. Wiestler (M.D.), President, Helmholtz society (from 2015); Director, DKFZ German cancer research center (until 2015), Heidelberg
Heinz Höfler (M.D.)
Head of Pathology, Technical University of Munich
Dieter Werner (Ph.D.)
lab head (retired) "Biochemistry of the Cell" at the Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg
In 2001 I developed my research concept which in my view and especially at that time was really great. Unfortunately, the collaborating biophysics lab was unable to support me as a long-year guest scientist with this project to the extend necessary to get this into Cell, Science or Nature.
Although I had several abstracts since 2001 with the lab, but none of my expected, perhaps five, manuscripts in preparation was close to be finished by the lab in 2003, I was able to submit in September 2003 the first AFM measurements of VLA-4/VCAM-1 on a living cell in a smaller journal - I was really upset about collaborating over years with a lab unable to focus on publishing my research concepts in first class journals. Without my paper, it would be harder to prove that I really was the first - and that the lab later just continued with my research concept and other collaborations that I initiated and organized by myself. To my surprise, the lab and the university still actively avoid mentioning my publication in a students' thesis, as well as on the official university webpage with the lab publications list.